(1989 Noren et al.) New building blocks for protein engineering
Reports the development of a new method that enables the site-specific incorporation of unnatural amino acids into proteins. Synthetic amino acids were incorporated by the use of a chemically acylated suppressor transfer RNA that inserted the amino acid in response to a codon that is normally interpreted as a “stop” instruction.
With link to abstract:
Noren CJ, Anthony-Cahill SJ, Griffith MC, Schultz PG. (1989) “A general method for site-specific incorporation of unnatural amino acids into proteins” Science 244:182-8