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(1988 Regan & DeGrado) A first success in de novo protein design

Reports the first design and biosynthesis of a protein with a sequence unlike any found in nature. The resulting structure, a four-helix bundle protein, was extraordinarily stable. Its design relied on a simple, repetitive structure and gave no detailed attention to the packing of internal side-chains. Subsequent studies showed that its internal structure was ill-defined (a so-called “molten globule” state). Later designs focused on the packing of internal side chains and produced proteins with stable, well-defined internal structures.

With link to abstract:

Regan L, DeGrado WF (1988) “Characterization of a helical protein designed from first principles” Science 241:976-8.